The 17^th edition of the JP (Japanese Pharmacopoeia) came into effect on April 1, 2016. Miglitol was added to the 17^th edition of the JP. According to the JP method, it is necessary for the system suitability to satisfy theoretical plate number of ≥ 5,000 and symmetry factor of ≤ 1.5, and for the system repeatability to satisfy relative standard deviation (RSD) of ≤ 1.0% (repeated six times). It was confirmed that the all conditions were satisfied when miglitol was analyzed using Asahipak NH2P-50 4E.

Sample : 20μL
1. Miglitol 1mg/mL

Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm) 


Eluent : (4.5mM KH2PO4+0.47mM Na2HPO4 aq.)/CH3CN=25/75 


Flow rate : 0.7mL/min 


Detector : UV(210nm) 


Column temp. : 35°C