Pectin is a heteropolysaccharide which has a backbone composed of α-1,4- glycosidic bound galacturonic acids; a majority of the carboxyl groups of galacturonic acids are esterified with methoxy group. Pectin is extracted from the endothelial of citrus or the pomace of apples with dilute acid. Pectin is used as a binder, suspending agent and adhesive. In this application, two pectin samples of different origin were analyzed using OHpak SB-806M HQ, a column for aqueous SEC column.
Sample : Pectin 0.1% each, 100μL
Columns : Shodex OHpak SB-806M HQ (8.0mmI.D. x 300mm) x 2
Eluent : 0.1M NaNO3 aq.
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 40°C