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Analysis of Trisaccharises (NH2P-40 3E)

The trisaccharides melezitose, raffinose, and maltotriose were separated using Asahipak NH2P-40 3E, a polymer-based amino column.

Sample : 0.1% each, 10μL
1. Melezitose
2. Raffinose
3. Maltotriose



Column : Shodex Asahipak NH2P-40 3E (3.0mmI.D. x 250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 0.35mL/min
Detector : Shodex RI
Column temp. : 30?