The imidazole dipeptides, anserine (dipeptide of histidine and β-alanine) and carnosine (dipeptide of 1-methylhistidine and β-alanine), are found in large concentrations in the meat of bonito, tuna, whale, and the breast meat of birds. The imidazole dipeptides show a strong antioxidant effect which is effective for reducing fatigue and anti-aging. In this application, carnosine, anserine, and their constituent amino acids were analyzed simultaneously using polymer-based amino column, Asahipak NH2P-50 4E. These five compounds can be separated completely within 20 minutes.

Sample : 20μL
1. β-Alanine 200μg/mL
2. 1-Methyl-L-histidine 2μg/mL
3?L-Anserine 5μg/mL
4. Histidine 5μg/mL
5. L-Carnosine 5μg/mL
6. Nitrate
(derived from L-Anserine Nitrate)

Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)

Eluent       : 50mM NaH2PO4 aq./CH3CN=40/60

Flow rate    : 1.0mL/min

Detector     : UV(210nm)

Column temp. : 40°C