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LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)

Pipecolic acid or piperidine-2-carboxylic acid was analyzed by Asahipak NH2P-40 2D a polymer-based amino column under HILIC mode. Pipecolic acid has been proposed as a diagnostic indicator of pyridoxine-dependent epilepsy. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for the high sensitivity analysis by LC/MS.

Sample : 5μL
L-Pipecolic acid 10ng/mL
(in H2O/CH3CN=25/75)



Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)
Eluent : 20mM HCOONH4 aq./CH3CN=20/80
Flow rate : 0.2mL/min
Detector : ESI-MS/MS SIM
Column temp. : 30°C