, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

Analysis of Zanamivir According to USP Method (NH2P-50 4E)

Zanamivir is a neuraminidase inhibitor and it is effective in the treatment of influenza caused by influenza A and B viruses. According to USP (United States Pharmacopeia) method, zanamivir should be analyzed using a column which can separate zanamivir and talo-zanamivir (internal standard) with resolution (Rs) of ≥ 1.5 and relative standard deviation (RSD) of ≤ 1.5%. It was confirmed that Rs was higher than 1.5 and RSD was lower than 1.5% when zanamivir and talo-zanamivir were analyzed using Asahipak NH2P-50 4E.

Sample : 20μL
Zanamivir resolution solution
1. Zanamivir 0.05mg/mL
2. talo-Zanamivir 2.5μg/mL


 


Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : Acetonitrile and 7.5 mM sulfuric acid (60:40). Adjust with ammonia TS to a pH of 6.2
Flow rate : 1.5mL/min
Detector : UV(234nm)
Column temp. : 30°C