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LC/MS Analysis of Creatinine and Creatine

Creatine and creatinine, the final metabolite of creatine, were separated by polymer-based amino column, Asahipak NH2P-40 2D, under HILIC mode. Hydrophilic compounds are retained at a high concentration acetonitrile under HILIC mode condition. This is advantageous for the high sensitivity analysis by ESI-MS.

Sample : 100ng/mL each, 5μL
1. Creatinine
2. Creatine


Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)
Eluent : (A); 50mM Ammonium formate aq. (B); CH3CN linear gradient; B%; 90% (0min)?E0% (6min)?E5% (7min)?E5% (20min)
Flow rate : 0.2mL/min
Detector : Creatinine; ESI-MS Creatine; ESI-MS/MS
Column temp. : 30°C