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LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)

Carnitine and Trimethyl glycine were analyzed by Asahipak NH2P-40 2D a polymer-based amino column under HILIC mode. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for high sensitivity analysis by LC/MS.

Sample : 5μL
5ng/mL each
(in H2O/CH3CN=25/75)
1. Trimethyl glycine
2. Carnitine



Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)
Eluent : 50mM HCOONH4 aq./CH3CN=20/80
Flow rate : 0.2mL/min
Detector : ESI-MS/MS SIM
Column temp. : 30°C