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Analysis of Pyridylaminated (PA) Monosaccharides

Pyridylaminated (PA) monosaccharides were analysed by Asahipak NH2P-50 4E. Carbohydrates are usually detected by refractive index detector (RI), but pyridylamination of saccharides enables high sensitivity analysis by fluorescence detector.

Sample : 5μL
1. 2-Aminopyridine, 0.1pmol/μL
2. PA-Rhamnose, 1pmol/μL
3. PA-Fucose, 1pmol/μL
4. PA-Ribose, 1pmol/μL
5. PA-Xylose, 1pmol/μL
6. PA-N-Acetylglucosamine, 1pmol/μL
7. PA-N-Acetylgalactosamine, 1pmol/μL
8. PA-Mannose, 1pmol/μL
9. PA-Glucose, 1pmol/μL
10. PA-Galactose, 1pmol/μL


Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : Phosphoric acid/H2O/CH3CN=1/14/85
Flow rate : 0.5mL/min
Detector : Fluorescence(Ex. 310nm, Em. 380nm)
Column temp. : 40°C