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LC/MS/MS Analysis of Saccharides (NH2P-40 2D)

A polymer based amino
Column, Asahipak NH2P-40 2D, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the
Eluent enhances the ionization of saccharides, thus providing high sensitivity detection.

Sample : 50ng/mL each, 5μL
1. meso-Erythritol
2. Xylitol
3. Fructose
4. Glucose
5. Sucrose
6. Lactose
7. Maltose
8. Raffinose

Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)

Eluent : (A) 0.05% NH3 aq. /(B) CH3CN Isocratic ; (B%) 75%

Flow rate : 0.2mL/min

Detector : ESI-MS/MS (MRM Negative) (m/z) 121→82(-) for meso-Erythritol, 151→59(-) for Xylitol, 179→89(-) for Fructose and Glucose, 341→59(-) for Sucrose, 341→161(-) for Lactose and Maltose, 503→89(-) for Raffinose

Column temp. : 40°C