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Stevioside and Rebaudioside A (NH2P-50 4E)

Polymer-based-amino column, Asahipak NH2P-50 4E was used to separate stevioside and rebaudioside A, that were extracted from stevia.

Sample : 0.05% each, 20μL
1. Stevioside
2. Rebaudioside A



Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : H2O/CH3CN=25/75
Flow rate : 1.0mL/min
Detector : UV(210nm)
Column temp. : 30°C