, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)

Pipecolic acid or piperidine-2-carboxlyic acid was analyzed by Asahipak NH2P-40 2D a polymer-based amino
Column under HILIC mode. Pipecolic acid has been proposed as a diagnostic indicator of pyridoxine-dependent epilepsy. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for the high sensitivity analysis by LC/MS.

Sample : 5μL
L-Pipecolic acid 10ng/mL
(in H2O/CH3CN=25/75)

Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)

Eluent : 20mM HCOONH4 aq./CH3CN=20/80

Flow rate : 0.2mL/min

Detector : ESI-MS (SIM Positive)

Column temp. : 30°C