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Comparison between KW402.5-4F and KW-802.5

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Protein analysis data using KW402.5-4F, high performance semi-micro aqueous SEC (GFC) column, and current PROTEIN KW-802.5. Theoretical plate number of KW400-4F series has improved 1.5 times than KW-800 series. Sensitivity becomes 3 or 4 times better than current column. KW400-4F series is suitable for high resolution and high sensitivity analysis.


Sample : 10μL
1. Blue dextran 2000, 0.2mg/mL
2. γ-Globulin, 0.8mg/mL
3. Ovalbumin, 0.8mg/mL
4. Myoglobin, 0.56mg/mL
5. Uridine, 0.04mg/mL


Columns      : (A); Shodex KW402.5-4F (4.6mmI.D. x 300mm)
               (B); Shodex PROTEIN KW-802.5 (8.0mmI.D. x 300mm)
Eluent       : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl
Flow rate    : (A); 0.33mL/min, (B); 1.0mL/min
Detector     : UV(280nm) (small cell volume)
Column temp. : Room temp.(25°C)