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Standard Proteins (7) (CM-825, SP-825)

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Six kinds of protien standard were analyzed using IEC CM-825 ( a column for weak cation exchange chromatography ) and IEC SP-825 ( a column for strong cation exchange chromatography ).


Sample :
1. Myoglobin, 2. Trypsinogen, 3. Ribonuclease A, 4. α Chymotrypsinogen A, 5. Cytochrome c, 6. Lysozyme

Columns      : (Left) Shodex IEC CM-825 (8.0mmI.D. x 75mm)
               (Right) Shodex IEC SP-825 (8.0mmI.D. x 75mm)
Eluent       : (A); 20mM Sodium phosphate buffer(pH7.0)
               (B); (A) + 0.5M NaCl
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.