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Proteins in Human Serum (2) (DEAE-825)

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Proteins in human serum were analyzed using IEC DEAE-825 (a column for weak anion exchange chromatography).


Sample : Human Serum
1. Albumin
2. α1 Acid glycoprotein
3. Transferrin
4. IgA
5. IgM
6. IgG

Column       : Shodex IEC DEAE-825 (8.0mmI.D. x 75mm)
Eluent       : (A); 20mM Tris-CH3COOH buffer(pH8.0)
               (B); (A) + 0.5M CH3COONa
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.