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Recovery of Proteins and Enzyme Activity (PH-814)

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Recovery of proteins and enzyme activity were measured using HIC PH-814 (a hydrophobic interaction column). With the exception of myoglobin, which had a recovery of 93%, all proteins show good recovery values. Enzyme activity for all proteins remained high, except for catalase which had a value of 88% and hexokinase which had a value of 94%.

Recovery of proteins
ProteinRecovery(%)
Cytochrome c104
Myoglobin93
Ribonuclease A100
Ovalbumin103
Lysozyme100
α-Chymotrypsin99
α-Chymotrypsinogen A96
Recovery of enzyme activity
EnzymeRecovery(%)
Trypsin96
Hexokinase94
Catalase88
Lysozyme100
Lipoxidase100


Sample load : 0.5mg each

Column : Shodex HIC PH-814