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Analysis of Peptide using Multimode Column (GS-320)

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Although Asahipak GS-HQ series is basically SEC columns, they permit different types of separation and analysis from conventional ones by use of a multimode which combines the hydrophobic interaction (RPC) and ionic interaction (IEX: ion exclusion). The GS-320 HQ column performs well in separating hydrophillic peptides, particularly acidic and basic peptides. As the columns can be used over a wide range of eluent pH because of being polymer-based column, they can be washed with alkaline solution leading to a longer column life, which is another feature of the column.

Sample : 20μL
1. Glu-Ala-Glu 0.025%
2. Arg-Asp 0.05%
3. Gly-His-Lys 0.025%
4. Arg-Pro-Lys-Pro 0.025%

 MWpIΣf
Glu-Ala-Glu
347
3.12
0.39
Arg-Asp
289
6.75
0.68
Gly-His-Lys
340
9.95
0.29
Arg-Pro-Lys-Pro
497
11.44
3.24
pI : Isoelectric point
Σf : Hydrophobic parameter
Column       : Shodex Asahipak GS-320 HQ (7.5mmI.D. x 300mm)
Eluent       : 50mM Ammonium acetate buffer(pH6.7)
Flow rate    : 0.5mL/min
Detector     : UV(220nm)
Column temp. : 30°C