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Histidine and 1-Methylhistidine (ODP-50)

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Histidine and 1-methylhistidine were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).


Sample : 0.005% each, 5μL
1. Histidine
2. 1-Methylhistidine
Column       : Shodex Asahipak ODP-50 6D (6.0mmI.D. x 150mm)
Eluent       : 10mM Na2HPO4 aq.
Flow rate    : 0.5mL/min
Detector     : UV(210nm)
Column temp. : 30°C