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Nucleobases, Nucleosides and Nucleotides (GS-320 HQ)

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Asahipak GS-320 HQ (a multimode column) is suitable not only for the separation of nucleobases, nucleosides and nucleotides but also for the separation of samples of their mixtures. GS-320 HQ permits one-time separation by isocratic elution without using gradient elution.


Sample : 20μL
1. ATP 100μg/mL
2. ADP 100μg/mL
3. IMP 100μg/mL
4. AMP 50μg/mL
5. GMP 100μg/mL
6. Inosine 100μg/mL
7. Adenine 100μg/mL
8. Hypoxanthine 100μg/mL
9. Guanine 100μg/mL
10. Adenosine 100μg/mL


Column       : Shodex Asahipak GS-320 HQ (7.5mmI.D. x 300mm)
Eluent       : 205mM NaH2PO4 aq./205mM H3PO4 aq.=300/7
Flow rate    : 0.6mL/min
Detector     : UV(260nm)
Column temp. : 30°C