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Sulfanilamides (DE-413)

Sulfanilamides were analyzed using RSpak DE-413, a
Column for reversed phase chromatography.

Sample : 20mg/L each, 10μL
1. Succinylsulfathiozole
2. Sulfadiazine
3. Sulfamerazine
4. Sulfathiazole
5. Sulfamethazine
6. Sulfanilamide

Column : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)

Eluent : 25mM Sodium phosphate buffer(pH7.6)/CH3CN/=82/18

Flow rate : 1.0mL/min

Detector : UV(254nm)

Column temp. : 40°C