Simultaneous Analysis of Saccharides and Sweeteners in Yogurt Drink (NH2P-40 2D)
Saccharides and sweeteners in yogurt (drink type) were analyzed using Asahipak NH2P-40 2D, a polymer-based amino column, with LC/MS/MS detection. Alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.
(Pretreatment of sample) 1) Add 3mL of CH3CN to 1mL of yogurt (drink type) and mix. 2) Centrifuge (3,000rpm x 5min) 3) Add 9.9mL of H2O/CH3CN=1/1 to 0.1mL of the supernatant and mix (= sample solution)