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Elution Volume of Aromatic Organic Acids (NN-814)

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RSpak NN-814, which has a higher polarity gel than that of RSpak KC-811 is suitable for the analysis of aromatic organic acids. The ion exclusion and reversed phase modes contribute to the separation of aromatic organic acids.


SubstanceElution Volume
(mL)
Shikimic acid
10.1
DL-Mandelic acid
19.2
Phthalic acid
19.8
Trimellitic acid
23.0
m-Hydroxybenzonic acid
46.5
Vanillic acid
47.1
p-Hydroxybenzoic acid
47.6
o-Hydroxybenzoic acid
48.8
3-Phenylpropionic acid
49.7
SubstanceElution Volume
(mL)
Benzoic acid
51.0
Caffeic acid
66.9
Phenylpropionic acid
68.4
Trimesic acid
69.0
Salicylic acid
71.4
m-Toluic acid
86.4
Ferulic acid
87.0
p-Coumaric acid
90.3


Column       : Shodex RSpak NN-814 (8.0mmI.D. x 250mm)
Eluent       : 0.1% H3PO4 aq.
Column temp. : 60°C