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Phenobarbital in Serum (GF-310 4B)

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Phenobarbital in serum was analyzed using a column for rapid analyses of medicines, MSpak GF-310 4B. First, large molecular weight portion, serum proteins was eluted, and then, phenobarbital which had interaction of adsorption and partition with packing material was eluted.


Sample : 2μL
5μL/mL Phenobarbital in Control serum I Wako
(from Wako Chemicals Indusries, Ltd.)
Column       : Shodex MSpak GF-310 4B (4.6mmI.D. x 50mm) 
Eluent       : 50mM CH3COONH4/CH3CN=70/30
Flow rate    : 0.25mL/min
Detector     : UV(220nm)
Column temp. : 30°C