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Recovery of Proteins (GF-510 HQ and GS-520 HQ)

Asahipak GF-510 HQ, GS-520 HQ permit GFC analysis with good recovery of proteins.

ProteinsRecovery (%)
GF-510 HQ GS-520 HQ
γ-Globulin
85
85
Transferrin
88
92
BSA
90
95
Lysozyme
95
96

Columns : Shodex Asahipak GF-510 HQ, GS-520 HQ (7.5mmI.D. x 300mm each)
Eluent : 0.1M Sodium phosphate buffer(pH7.0) + 0.1M NaCl
Flow rate : 1.0mL/min
Detector : UV(210nm)
Column temp. : 30°C