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Hippuric Acid etc. in Urine (2) (DE-413)

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Some metabolites in urine have been added to the health check as special items in Japan recently. From the items, hippuric acid, methylhippuric acid(o-, m-, p-), mandelic acid which are metabolites of toluene, xylene and styrene and creatinine were analyzed using RSpak DE-413 (a column for reversed phase chromatography) within 10 minutes.


Sample : 10μL
(Left); Standards
1. Creatinine 20mg/L
2. Mandelic acid 10mg/L
3. Hippuric acid 10mg/L
4. o-Methylhippuric acid 10mg/L
5. p-Methylhippuric acid 10mg/L
6. m-Methylhippuric acid 10mg/L

(Right); Urine 100-fold diluted
Column       : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)
Eluent       : CH3CN/Buffer=1/5
               (Buffer: 20mM Sodium phophate + 20mM β-Cyclodextrin
                + 4.5mM sodium dodecyl sulfate(pH3.2))
Flow rate    : 1.0mL/min
Detector     : UV(225nm)
Colomn temp. : 50°C