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Chiral Separation of Phenylalarine (CRX-853)

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A column for chiral separation, ORpak CRX-853, was used to separate D and L isomers of phenylalarine.


Sample : DL-Phenylalanine 0.05%, 50μL
1. D-Phenylalanine
2. L-Phenylalanine


Column       : Shodex ORpak CRX-853 (8.0mmI.D. x 50mm)
Eluent       : 8mM CuSO4 aq./CH3CN=80/20 
Flow rate    : 1.0mL/min
Detector     : UV(230nm)
Column temp. : 50°C