, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

Analysis of VMA and Creatinine using Column Switching Method

Vanillylmandelic acid (VMA) and creatinine in urea are determined by fluorescent detection using three columns, Asahipak ES-502N, GS-320 and NH2P-50 and column-switching method. GS-320 7E is an old type of GS-320 HQ.



Sample : a. Diluted urine , b. Standard VMA and Creatinine


Columns : Shodex Asahipak ES-502N 7C (7.5mmI.D. x 100mm), GS-320 7E (7.5mmI.D. x 250mm), NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : 0.35mol Propionic acid + 0.3mol NaOH in 1kg H2O(pH3.75)
Flow rate : 0.7mL/min
Detector : Fluorescence
Detector(Ex: 280nm, Em: 315nm)

Courtesy of Dr. Seki, Osaka University