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Simultaneous Analysis of Water-Soluble Vitamins (DE-413L)

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Ten kinds of water-soluble vitamins were simultaneously analyzed using RSpak DE-413L, a polymer-based reversed phase chromatography column.


Sample : 5μL
(in 250mM H3PO4 aq.)
1. Vitamin B1 200µM
2. Vitamin B6 200µM
3. Niacinamide 200µM
4. Vitamin C 200µM
5. Vitamin B3 200µM
6. Vitamin B5 200µM
7. Vitamin B12 200µM
8. Folic acid 20µM
9. Vitamin B2 200µM
10. Biotin 400µM


Column       : Shodex RSpak DE-413L (4.6mmI.D. x 250mm)
Eluent       : (A); 10mM H3PO4 aq./(B); CH3CN 
               High pressure linear gradient; 
               (B%) 0% to 30% (0 to 5min), 30% (5 to 10min), 0% (10 to 20min)
Flow rate    : 1.0mL/min
Detector     : PDA (190-400nm)
Column temp. : 50°C