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Analysis of Polyamines by On-Column Derivertization

Polyamines were analyzed by on-column derivertization using Asahipak ODP-50 4D.
Refer to

Sample : 10μL
Histamine (Him) , Diethylenetriamine (Det:I.S.) , Tyramine (Tym) , 1,3-Diaminopropane (Dap)
Putrescine (Put) , Spermidine (Spd) , Spermine (Spm) , Cadaverine (Cad)



Column : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm)
Eluent : 50mM Borate buffer (pH9.9) - CH3CN(77:23,v/v) containing 2mM OPA(o-Phthalaldehyde) and 2mM NAC(N-Acetyl-L-cysteine)
Flow rate : 0.5mL/min
Detector : Fluorescence(Ex. 330nm, Em. 430nm)
Column temp. : 40°C

K.Saito, M.Horie, N.Nose,K.Nakagomi and H.Nakazawa, Anal. Sci. 8,675(1992)