, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

Effects of Temperature on GPC Clean-up (EV-2000 AC)

Print

Two different temperature settings (room temerature and 40°C) were compared for the GPC clean-up method using CLNpakEV-2000 AC . At room temperature, the high viscosity causes peak broadening and increases the analysis time. Therefore, analysis at 40°C is preferred. In Food Sanitation Law #1003001 (Released by Ministry of Health, labour and welfare in Japan, 2006), analysis temperature is set at 40°C.


Sample : 5mL
Fatty tuna
Acrinathrin 40mg/L
Tricyclazole 40mg/L
Column      : Shodex CLNpak EV-G AC (20.0mmI.D. x 100mm) + EV-2000 AC (20.0mmI.D. x 300mm)
Eluent      : Acetone/Cyclohexane=1/4
Flow rate   : 5mL/min
Detector    : UV(254nm)