Effects of Temperature on GPC Clean-up (EV-2000 AC)
Two different temperature settings (room temerature and 40°C) were compared for the GPC clean-up method using CLNpakEV-2000 AC . At room temperature, the high viscosity causes peak broadening and increases the analysis time. Therefore, analysis at 40°C is preferred. In Food Sanitation Law #1003001 (Released by Ministry of Health, labour and welfare in Japan, 2006), analysis temperature is set at 40°C.