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LC/MS/MS Analysis of Creatinine and Creatine (NH2P-40 2D)

Creatine and creatinine, the final metabolite of creatine, were separated by polymer-based amino
Column, Asahipak NH2P-40 2D, under HILIC mode. Hydrophilic compounds are retained at a high concentration acetonitrile under HILIC mode condition. This is advantageous for the high sensitivity analysis by ESI-MS/MS.

Sample : 5μL
10ng/mL each
(in H2O/CH3CN=1/3)
1. Creatinine
2. Creatine
3. Guanidinoacetic acid

Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)

Eluent : 20mM HCOONH4 aq./CH3CN=30/70

Flow rate : 0.2mL/min

Detector : ESI-MS/MS(MRM Positive)

Column temp. : 30°C