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Effects of Flow Rate (1) (HK-404L)

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The general flow rate of a semi-micro column (4.6mm I.D.) is 0.3mL/min. The eluent can pass through HK-404L column at a higher flow rate with the conventional HPLC instrument. Polystyrene was analyzed using a flow rate of 0.3mL/min and 1.0mL/min. When the flow rate was raised to 1.0mL/min, the molecular chains were not degraded.


Sample : Polystyrene


Column       : Shodex GPC HK-404L (4.6mmI.D. x 150mm)
Eluent       : THF
Detector     : UV(254nm) (small cell volume)
Column temp. : 40°C