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Anions and Organic Acids (NI-424)

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IC NI-424 (an anion analysis column for non-suppressor method) has high theoretical plate number (more than 5,000). Carbonate and other anions can be separated well with NI-424.


Sample : 20μL
1. Acetic acid 20.0mg/L
2. Formic acid 20.0mg/L
3. Malic acid 20.0mg/L
4. Tartaric acid 20.0mg/L
5. Oxalic acid 20.0mg/L
6. H2PO4- 20.0mg/L
7. F- 1.5mg/L
8. Cl- 2.5mg/L
9. NO2- 6.0mg/L
10. Br- 10.0mg/L
11. NO3- 10.0mg/L
12. SO42- 10.0mg/L


Column       : Shodex IC NI-424 (4.6mmI.D. x 100mm)
Eluent       : 8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris + 2mM Phenylboronic acid 
               + 0.005mM *CyDTA aq.
Flow rate    : 1.0mL/min
Detector     : Non-suppressed conductivity
Column temp. : 40°C
* CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid