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Water-Soluble Vitamins (4)

Six vitamines and caffein were separated isocratically using RSpak DM-614.

Sample :
1. Vitamin C
2. Vitamin B13
3. Vitamin B6
4. Vitamin B1
5. Vitamin B3
6. Caffeine



Column : Shodex RSpak DM-614 (6.0mmI.D. x 150mm)
Eluent : 0.055M Na2HPO4 + 0.045M KH2PO4 aq.
Flow rate : 1.0mL/min
Detector : UV(254nm)
Column temp. : 28°C