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Vitamin E

Silica 5SIL 4D is suitable for the separation of tocopherols. The chromatogram on the left shows the separation of α-, β-, γ- and δ-tocopherol by normal phase mode using the column with a mixture of n-hexane/isopropanol as an eluent. Asahipak ODP-50 6D is also suitable for the separation of tocopherols though β- and γ-tocopherol can not be separated with the column. The chromatogram on the right shows the separation of α-, β-, and γ-tocopherol by reversed phase mode using the column with a methanol eluent.

Sample : Tocopherol
1. α-Tocopherol
2. β-Tocopherol
3. γ-Tocopherol
4. δ-Tocopherol


              (left)                                (right)

Column : Shodex Silica 5SIL 4D Shodex Asahipak ODP-50 6D (4.6mmI.D. x 150mm) (6.0mmI.D. x 150mm)
Eluent : n-Hexane/Isopropanol=99.5/0.5 CH3OH
Flow rate : 1.0mL/min 1.0mL/min
Detector : UV(280nm) UV(295nm)
Column temp. : 30°C 30°C