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Column Test Conditions (Hydrophobic Interaction Chromatography Column)

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To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be calculated by the formula in the following figure.



Column typeTest conditions
(1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
PH-814(1)0.5% Acetone (2)20μL (3)H2O (4)1.0mL/min (5)Ambient (6)UV(280nm)