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Column Test Conditions (Ion Chromatography Columns)


To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be calculated by the formula in the following figure.

Column typeTest conditions
(1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
YS-50(1)20mg/L Ca2+ (2)10μL (3)4mM Methanesulfonic acid aq. (4)1.0mL/min (5)40°C (6)CD
YK-421(1)20mg/L Ca2+ (2)15μL (3)5mM Tartaric acid + 1mM Dipicolinic acid + 24mM Boric acid aq. (4)1.0mL/min (5)40°C (6)CD
Y-521(1)20mg/L Na+ (2)50μL (3)4mM HNO3 aq. (4)1.5mL/min (5)40°C (6)CD
T-521(1)20mg/L Na+ (2)20μL (3)3mM HNO3 aq. (4)1.0mL/min (5)40°C (6)CD
NI-424(1)10mg/L SO42- (2)50μL (3)8mM 4-Hydroxybenzoic acid + 2.8mM Bis(2-hydroxyethyl)imino- tris(hydroxymethyl)methane + 2mM Phenylboronic acid + 5μM trans-1,2-Aminocyclohexane-N,N,N',N'-4 acetic acid aq.(4)1.0mL/min (5)40°C (6)CD
I-524A(1)10mg/L NO3- (2)30μL (3)2.5mM Phthalic acid aq.(pH4.0) (4)1.5mL/min (5)40°C (6)CD
SI-90 4E (1)15mg/L SO42- (2)20μL (3)1.8mM Na2CO3 + 1.7mM NaHCO3 aq. (4)1.0mL/min (5)Ambient (6)Suppressed CD
SI-50 4E (1)10mg/L Br- (2)20μL (3)3.2mM Na2CO3 + 1.0mM NaHCO3 aq. (4)0.7mL/min (5)Ambient (6)Suppressed CD
SI-52 4E (1)40mg/L SO4- (2)50μL (3)3.6mM Na2CO3 aq. (4)0.8mL/min (5)45°C (6)Suppressed CD
SI-35 4D (1)40mg/L SO4- (2)20μL (3)3.6mM Na2CO3 aq. (4)0.6mL/min (5)45°C (6)Suppressed CD