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Leukotrienes Produced by Polymorphonuclear Leukocyte of Rat

Leukotrienes produced by polymorphonuclear leukocyte of rat were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).

Sample : Leukotrienes



Column : Shodex Asahipak ODP-50 6D (6.0mmI.D. x 150mm)
Eluent : 0.2M CH3COONa buffer(pH4.5 adjusted with CH3COOH)/CH3CN/CH3OH=50/25/25
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : 30°C

Courtesy of Dr. Oishi and Dr. Yamaki, Kitazato University