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Comparison of Chromatograms of Protein mixtures

This figure shows the chromatogram of protein mixtures analyzed with OHpak SB-803 HQ, Asahipak GF-510 HQ or PROTEIN KW-803. KW-803 is the best column for the separetion of these proteins.


Sample :
A: Thyroglobulin (bovine)
B: γ-Globulin (bovine)
C: Ovalbumin (chiken)
D: Myoglobin (horse)
E: Cyanocobalamin


Columns : Shodex OHpak SB-803 HQ (8.0mmI.D. x 300mm) Shodex Asahipak GF-510 HQ (7.5mmI.D. x 300mm) Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm)
Eluent : 0.2M Phosphate buffer(pH6.9)
Flow rate : 0.5mL/min
Detector : UV(280nm)
Column temp. : 30°C