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High Sensitive Analysis of Metalloproteins in Lat Liver Cell

High sensitive analysis of metalloproteins in lat liver cell was performed using GS320A-M5D (0.5mm I.D. x 150mm) and ICP-MS. GS320A-M5D is a micro column of Asahipak GS-320 HQ.

Sample : 100nL
Cu,Zn-SOD (Super oxide dismutase)
Cu-MT (Metallothionein)



Column : Shodex GS320A-M5D (0.5mmI.D. x 150mm)
Eluent : 50mM Ammonium acetate buffer(pH6.5)
Flow rate : 2.0μL/min
Detector : ICP-MS(Cu and Zn at m/z=65 and 66,respectively)

Courtesy of Dr. Yasumitsu Ogra, Graduate School of Pharmaceutical Sciences, Chiba University