, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

LC/MS Analysis of Melamine and Cyanuric Acid

Cyanuric acid is a structural analogue of melamine, and thus sometimes detected as an impurity of melamine. In this application, polymer-based amino column, Asahipak NH2P-40 2D , was used to separate melamine and cyanuric acid using HILIC mode. Hydrophilic compounds are retained at a high concentration acetonitrile under HILIC mode condition. This is advantageous for the high sensitivity analysis by ESI-MS.

Sample : 500ng/mL each, 5μL
1. Melamine
2. Cyanuric acid



Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)
Eluent : 50mM Ammonium formate aq./CH3CN=25/75
Flow rate : 0.2mL/min
Detector : Melamine; ESI-MS/MS Cyanuric acid; ESI-MS
Column temp. : 30°C