RSpak RP18-415 (a column for reversed phase chromatography) can analyze a variety of proteins with good recovery. This column is suitable for fast analysis at high flow rates since the maximum working pressure is 22Mpa.
Recovery of Proteins
1. Ribonuclease A
3. Cytochrome c
Column : Shodex RSpak RP18-415 (4.6mmI.D. x 150mm)
Eluent : (A); 0.1% TFA aq./CH3CN=99/1
(B); 0.1% TFA aq./CH3CN=5/95
Linear gradient: 0 to 25min, 20% (B) to 60% (B)
Flow rate : 1.0mL/min
Detector : UV(220nm)
Column temp. : Room temp.