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Standard Proteins (3) (RP18-415)

RSpak RP18-415 (a column for reversed phase chromatography) can analyze a variety of proteins with good recovery. This column is suitable for fast analysis at high flow rates since the maximum working pressure is 22Mpa.

Recovery of Proteins
1. Ribonuclease A
93%
2. Insulin
98%
3. Cytochrome c
100%
4. Lysozyme
100%
5. BSA
98%
6. Myoglobin
108%
7. Ovalbumin
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Column : Shodex RSpak RP18-415 (4.6mmI.D. x 150mm)
Eluent : (A); 0.1% TFA aq./CH3CN=99/1 (B); 0.1% TFA aq./CH3CN=5/95 Linear gradient: 0 to 25min, 20% (B) to 60% (B)
Flow rate : 1.0mL/min
Detector : UV(220nm)
Column temp. : Room temp.