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Influence of
Column Temperature on Retention Time of PEG (GF-310 HQ and GS-320 HQ)

Poly(vinyl alcohol) gel with different hydrophobicity are packed into Asahipak GF-310 HQ and GS-320 HQ
Columns. The hydrophobicity of the gel of the two
Columns is going stronger according to the temperature rising. Such influence is observed stronger with GF-310 than GS-320. Then under using water as the
Eluent at 50 °C, PEG is not eluted according to its molecular weight as shown the figure. In order to avoid such influence of hydrophobicity, it is applicable to make close the hydrophobicity of gel and
Eluent. For example the addition of organic solvent, such as Methanol, to
Eluent is in effect.
Please refer to .

Sample :
1. PEG, Poly(ethylene glycol) (MW: 20,000)
2. PEG, Poly(ethylene glycol) (MW: 4,000)
3. PEG, Poly(ethylene glycol) (MW: 1,000)
4. PEG, Poly(ethylene glycol) (MW: 400)
5. EG, Ethylene glycol (MW: 62)

Columns : Shodex Asahipak GF-310 HQ or GS-320 HQ (7.5mmI.D. x 300mm each)

Eluent : H2O

Flow rate : 0.5mL/min

Detector : Shodex RI

Column temp. : 30°C or 40°C or 50°C