When analyzing saccharides in food, it is necessary to remove the substances besides saccharides before analysis. This procedure is called as sample pre-treatment. Here, the analysis result of saccharides in food using Asahipak NH2P-50 4E is shown together with the sample pre-treatment procedure. (Sample pretreatment) 1) Take 5g of sugar added yogurt in a beaker, add 30mL of pure water and neutralize with 10%(w/v) NaOH. 2) Apply ultrasonic vibration for 30 minutes and make 50 mL solution. 3) Filtrate with No.5B paper filter and then with 0.45μm membrane filter. The comparison of NH2P-50 column and silica-based amino column for the analysis of yogurt(sugar added) is as follows: 1)The peaks of fructose and galactose cannot be detected using silica-based amino column. 2)In case of NH2P-50 column, the peak of lactose is lager compared with the case of silica-based amino column. This result shows the fact that most of lactose is adsorbed by silica-based amino column. Since the adsorption of lactose by NH2P-50 column is not so much, the detection limit of NH2P-50 column is lower than that of silica-based amino column.