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Sample Load (KW-803)

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Shodex PROTEIN KW-803 (a column for aqueous SEC (GFC)) shows good efficiency until 0.5mg of BSA (Bovine serum albumin) injection. If the injection to KW-803 exceeds 1mg, chromatogram of BSA becomes gradually broad. Recommended sample loading is less than 0.5mg of BSA per column.


Sample : BSA, 100μL


Column       : Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm)
Eluent       : 50mM Phosphate buffer(pH7.0) + 150mM NaCl
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.