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Calibration Curves for GF-HQ Series (Aqueous Eluent: Proteins)

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Calibration curves for Asahipak GF-510 HQ and GF-7M HQ (Aqueous/Organic (multi-solvent use) SEC columns) are shown.


Sample:
1. IgM
2. IgA
3. IgG
4. Transferrin
5. Conalbumin
6. BSA
7. Ovalbumin
8. Myoglobin
9. Ribonuclease A
10. Cytochrome c
11. L-Phenylalanin
12. Creatinine


Columns      : Shodex Asahipak GF-510 HQ            : GF-7M HQ
               (7.5mmI.D. x 300mm)                    (7.5mmI.D. x 300mm)
Eluent       : 0.1M Sodium phosphate buffer(pH7.0)  : 0.2M Sodium phosphate
               + 0.1M NaCl                            buffer(pH7.0)
Flow rate    : 0.5mL/min                            : 0.5mL/min
Detector     : UV(280nm)                            : UV(280nm)
Column temp. : 30°C                                 : 30°C