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Having high resolution, RSpak RP18-415 is suitable for the analysis of complex sample mixtures. Its performance is excellent in determining amino acid sequences in triptic digests of myoglobin. Sample : Triptic digests of Myoglobin
Column : Shodex RSpak RP18-415 (4.6 mm I.D. x 150 mm)
Eluent : (A); 0.1 % TFA aq./CH3CN=99/1
(B); 0.1 % TFA aq./CH3CN=5/95
Linear gradient: 0 to 30 min, (A) to 50 % (B)
Flow rate : 0.6 mL/min
Detector : UV (220 nm)
Column temp. : Room temp.