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Recovery of Proteins and Enzyme Activity (DEAE-825)

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The tables show good recovery of IEC DEAE-825 (a column for ion exchange chromatography) for proteins and enzyme activity.

Recovery of proteins
ProteinRecovery (%)
Trypsin inhibitor 96
β-Lactoglobulin 100
Ovalbumin 101
Myoglobin 100
γ-Globulin 99
BSA 97
Thyroglobulin 90
Recovery of enzyme activity
EnzymeRecovery (%)
Kallikrein 92
Lipoxidase 96
Catalase 94

Sample load : 0.5mg each

Column : Shodex IEC DEAE-825 (8.0mmI.D. x 75mm)
Eluent : (A); 20mM Tris-HCl buffer(pH8.0)
         (B); (A) + 0.5M NaCl
         Linear gradient: 0min to 1min, (A) to (B)