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Guanine and Tryptophan (DM-614, NN-814 and DE-613)

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Guanine and tryptophan were analyzed using three columns for reversed phase chromtography, RSpak DM-614, NN-814 and DE-613.


Sample : 1. Guanine, 2. Tryptophan


Columns     : (left);   Shodex RSpak DM-G 4A (4.6mmI.D. x 10mm) 
                        + DM-614 (6.0mmI.D. x 150mm)
              (Center); Shodex RSpak NN-G (6.0mmI.D. x 50mm) 
                        + NN-814 (8.0mmI.D. x 250mm)
              (Right);  Shodex RSpak DE-G 4A (4.6mmI.D. x 10mm) 
                        + DE-613 (6.0mmI.D. x 150mm)
Eluent      : (Left);   0.3M K2HPO4(pH8.6)  
              (Center); 0.1M NaH2PO4(pH3.0 adjusted by H3PO4)
              (Right);  0.23M K2HPO4 aq./CH3OH=80/20
Flow rate   : (Left); 0.8mL/min, (Center); 1.0mL/min, (Right); 0.8mL/min
Detector    : (Left); UV(278nm), (Center); Shodex RI, (Right); UV(278nm)
Column temp.: (Left); 30°C, (Center); 50°C, (Right); 30°C