Analysis of Tagatose According to USP-NF Method (SC1011)
According to USP40-NF35 method, the assay of tagatose should be used a column packed with L22. It is necessary for the system suitability to satisfy relative standard deviation (RSD) of ≤ 2.0%. It was confirmed to satisfy the system suitability when tagatose was analyzed using SUGAR SC1011.
Sample : 20 µL 1. Tagatose 5 mg/mL
Column : Shodex SUGAR SC1011 (8.0 mm I.D. x 300 mm)
Eluent : 0.05 mg/mL Calcium acetate aq.
Flow rate : 0.6 mL/min
Detector : Shodex RI
Column temp. : 85 °C